Efficient FLP recombination in mouse ES cells and oocytes.

نویسندگان

  • J Schaft
  • R Ashery-Padan
  • F van der Hoeven
  • P Gruss
  • A F Stewart
چکیده

We report an improved vector, pCAGGS-FLPe, for transient expression of the enhanced FLP recombinase in mouse ES cells and oocytes. In standard transfection experiments, about 6% of total ES colonies showed FLP recombination, albeit with mosaicism within each colony. After microinjection of pCAGGS-FLPe into oocytes, about one-third of heterozygotic mice born showed complete FLP recombination. Thus pCAGGS-FLPe presents two practical options for removal of FRT cassettes in mice.

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عنوان ژورنال:
  • Genesis

دوره 31 1  شماره 

صفحات  -

تاریخ انتشار 2001